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KMID : 0358619940280020143
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Korean Journal of Physiology
1994 Volume.28 No. 2 p.143 ~ p.150
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[ Current in Gastric Antral Myocytes
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Lee Moo-Yeol
Bang Hyo-Weon
Uhm Dae-Yong
Rhee Sang-Don
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Abstract
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The whole-cell mode of the patch clamp technique was used to study in gastric antral myocytes. Extracellular application of caffeine evoked . In order to isolate the chloride current from background current, all known systems were blocked with specific blockers. The current-voltage relationship of caffeine-induced current showed outward rectification and it reversed at around . The shift of reversal potential upon the alteration of external and internal chloride concentrations was well fitted with results which were calculated by the Nernst equation. Extracellular addition of N-phenylanthranilic acid and niflumic acid which are known anion channel blockers abolished the caffeine induced current. Intracellular application of a high concentration of EGTA also abolished this current. Application of c-AMP, c-GMP, heparin, or made no remarkable changes to this current. Sodium replacement with the impermeable cation N-methylglucamine or with rarely affected this current. From the above results it is suggested that the caffeine induced current was a current and it was activated by intracellular .
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KEYWORD
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Patch-clamp technique, current, Gastric antral myocyte
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